Analysis Note

Negative ControlHL-60 cells

Positive ControlA431 cells

Application

Immunoprecipitation (1-2 µg per sample) r>Neutralization Studies (2 µg/ml, see comments and application references)r>

General description

Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.

Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.

Immunogen

partially purified, human EGFR from A431 cells

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hunter T. 1984. Nature311, 414.Reynolds, F.H., et. al 1981. Nature292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.

Reconstitution

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)